Journal: Journal of Cellular and Molecular Medicine

Article Title: Ultrastructure damage of oviduct telocytes in rat model of acute salpingitis

doi: 10.1111/jcmm.12548

Figure Lengend Snippet: TCs immunodiagnostics by double-labelled immunofluorescence. Dotted arrows indicated CD34-positive vascular endothelial cells. Negative c-kit staining was not shown here; scale bar = 20 μm. (A) CD34 (red) in moniliform cells overlying vimentin (green) cells with DAPI counterstaining (blue) in sham control (solid arrows), indicated the existence of perivascular TCs with special immunophenotype of CD34/vimentin double-positive. (B and C) CD34/vimentin double-positive cells with specific TCs morphology and well-defined nuclei was significantly less densely stained, reduced, sparse or completely absent (solid arrows) in AS-affected oviduct tissues. A statistically significant decrease in the mean number of TCs occurred ( P = 0.000). * P < 0.05 versus sham control; error bars = SD.

Article Snippet: Then FITC-goat antimouse IgG for vimentin (1:50; cat no. BA1101), CY3-goat anti-rabbit IgG for CD34 (1:50; cat no. BA1032), TRITC-goat anti-rabbit IgG for c-kit (1:50; cat no. BA1090) were added (all from Boster).

Techniques: Immunofluorescence, Staining, Control

Journal: Journal of Cellular and Molecular Medicine

Article Title: Ultrastructure damage of oviduct telocytes in rat model of acute salpingitis

doi: 10.1111/jcmm.12548

Figure Lengend Snippet: TCs and Tps damage in AS-affected oviduct tissues, accompanied by excessive amount of collagen fibres (Coll) and tissue fibrosis. (A) Intercellular connection between damaged TCs and activated mononuclear cells (MC). (a) Degenerated Tps established closed contact to activated MC which contained dense secretory granules, together with granulocyte infiltration, mainly eosinophils (Eo) and neutrophils (PMN). b higher magnification of the boxed area; (b) synapse (black arrows) between activated MC and degenerated Tps, which contained lots of swollen mitochondria (m) and vacuoles (white arrows), thus indicating degeneration, functional insufficiency of TCs and involvement of TCs in local immunoregulation. (B) Degeneration, discontinue or dissolution of TCs and Tps (black arrows), with cytoplasmic vacuolization (white arrows), accompanied by nearly normal scattered putative stem cells (SCs). Intercellular contacts between TCs and SCs was getting wider or disappeared (black asterisks). (C) Disrupted TC-SC niches which composed of a group of damaged Tps and putative SCs in myosalpinx, with heterocellular contacts getting wider or disappeared between Tps and SCs (black asterisks), Tps and activated Eosinophils (Eo) (white asterisk) with dense secretory granules respectively. Degeneration, discontinue or dissolution of TCs and Tps (black arrows), with swollen mitochondria (m), cytoplasmic vacuolization (white arrows) in Tps, swollen and dissolution of SMCs can be observed. (D) Severely damaged perivascular TCs and Tps, with swollen mitochondria (m), rough endoplasmic reticulum (rER) dilatation and cytoplasmic vacuolization (white arrows), together with damaged endothelial cell (E) and pericytes (P).

Article Snippet: Then FITC-goat antimouse IgG for vimentin (1:50; cat no. BA1101), CY3-goat anti-rabbit IgG for CD34 (1:50; cat no. BA1032), TRITC-goat anti-rabbit IgG for c-kit (1:50; cat no. BA1090) were added (all from Boster).

Techniques: Functional Assay, Dissolution

Journal: Scientific Reports

Article Title: New lymphatic cell formation is associated with damaged brain tissue clearance after penetrating traumatic brain injury

doi: 10.1038/s41598-021-89616-3

Figure Lengend Snippet: Immunohistochemical staining images showing CD34 expression in ( A ) normal mouse brain tissue and ( B – F ) mouse brain tissue on days 3, 7, 15, 21 and 30 days, following the pTBI. ( A ) CD34 was not expressed in normal mouse brain tissue. CD34-positive cells and blood vessels (brown; black arrows) became visible at the injury site (red arrow) on days ( B ) 3 and ( C ) 7. ( D ) After 15 days, CD34 expression at the injury site (red arrow) decreased. ( E ) CD34 expression at the injury site could not be detected on day 21. Instead, black hemosiderin particles (black arrows) were observed at the injury site. ( F ) Some black hemosiderin particles remained visible at the injury site on day 30. ( G ) Immunohistochemical results of CD34 were analyzed by Image-Pro Plus 6.0 software. The average density (IOD) of CD34-positive cells in normal mouse brain tissue and in mouse brain tissue on days 3, 7, 15, 21 and 30 days, following pTBI. The n = 10/group was used for calculating the average IOD. Comparisons between groups were performed using the Kruskal–Wallis H test followed by Bonferroni post-hoc analysis.

Article Snippet: The sections were first incubated with rabbit anti-mouse PROX1 antibody (1:100; Boster Biological Technology Co., Wuhan, China), rabbit anti-mouse CD34 antibody (1:100; BosterBiological Technology) or goat anti-mouse LYVE-1 antibody (1:100; R&D Systems, Shanghai, China) at 4 °C for 24 h. Next, the sections were incubated with horseradish peroxidase-labeled goat anti-rabbit IgG or rabbit anti-goat IgG secondary antibodies.

Techniques: Immunohistochemical staining, Staining, Expressing, Software

Journal: Scientific Reports

Article Title: New lymphatic cell formation is associated with damaged brain tissue clearance after penetrating traumatic brain injury

doi: 10.1038/s41598-021-89616-3

Figure Lengend Snippet: Double immunofluorescence staining images showing CD34/LYVE-1 expression in the brain tissue 3 days after pTBI. Expression of ( A ) LYVE-1 (green); ( B ) CD34 (red); and ( C ) merge (yellow).

Article Snippet: The sections were first incubated with rabbit anti-mouse PROX1 antibody (1:100; Boster Biological Technology Co., Wuhan, China), rabbit anti-mouse CD34 antibody (1:100; BosterBiological Technology) or goat anti-mouse LYVE-1 antibody (1:100; R&D Systems, Shanghai, China) at 4 °C for 24 h. Next, the sections were incubated with horseradish peroxidase-labeled goat anti-rabbit IgG or rabbit anti-goat IgG secondary antibodies.

Techniques: Double Immunofluorescence Staining, Expressing

Journal: Stem Cell Research & Therapy

Article Title: Growth differentiation factor 11 alleviates oxidative stress-induced senescence of endothelial progenitor cells via activating autophagy

doi: 10.1186/s13287-024-03975-y

Figure Lengend Snippet: Rat bone marrow-derived CD34 + VEGFR-2 + EPCs. ( A ) Analysis of CD34 + VEGFR-2 + EPCs by flow cytometry. ( B ) CD34 + VEGFR-2 + EPCs (arrows) in the mononuclear cells isolated from bone marrow. Immunostaining. Scale bar = 25 μm. ( C ) CD31 expression in CD34 + VEGFR-2 + EPCs at 2 weeks after induction with VEGF. Immunostaining. Scale bar = 10 μm. ( D , E ) ALK-4 expression in EPCs demonstrated with immunostaining and Western blotting respectively. Scale bar = 25 μm. ( F ) The statistical result of ALK-4 expression. * P < 0.01 versus young group. n = 3

Article Snippet: The cells were then incubated with mouse anti-rat CD34 and rabbit anti-rat VEGFR-2 antibodies (1:100; Santa Cruz Biotech, Dallas, TX, USA) at 4 o C for 50 min, followed by incubation with Alexa Fluor 647-conjugated goat anti-mouse IgG and Alexa Fluor 488-conjugated goat anti-rabbit IgG (1:200; Jackson, West Grove, PA, USA) at 4 o C for 30 min. CD34 + VEGFR-2 + cells in the mononuclear cells were sorted using a Beckman MoFloTM XDP FACS (fluorescence-activated cell sorter; Beckman Coulter, Fullerton, CA, USA).

Techniques: Derivative Assay, Flow Cytometry, Isolation, Immunostaining, Expressing, Western Blot

Journal: PLoS ONE

Article Title: Antitumor and Angiostatic Activities of the Antimicrobial Peptide Dermaseptin B2

doi: 10.1371/journal.pone.0044351

Figure Lengend Snippet: Treatment of xenografted nude mice was started thirteen days after s.c. PC3 cell injection. Experimental group (n = 8) was treated six times per week p.t. with 100 µL Drs B2 (2.5 mg/kg body weight). Control group (n = 7) was treated six times per week p.t. with 100 µL of PBS. A ) The effect of Drs B2 on tumor size versus time of treatment. After 47 days of treatment the mice were sacrificed, body weight was observed; tumors were isolated, B ) weighted and stored at −80°C. C ) PC3 tumor proliferation was evaluated by Ki67 staining of frozen tissue sections. Proliferation was quantified by image J software analysis of Ki67 positive stained cells on the whole tumor section. The data are mean areas ± SD obtained from 6 control mice and 4 mice treated with Drs B2. Scale bar, 50 µm. * p <0.05 versus control (PBS). D ) Tumor vessel formation was observed with anti CD34 antibodies. Angiogenesis was quantified by image analysis of CD34 positive stained endothelial cells. The data are mean areas ± SD obtained from 6 control mice and 4 mice treated with Drs B2. Scale bar, 50 µm * p <0.05 versus control (PBS).

Article Snippet: Mouse monoclonal anti-CD34 was from Hycult Biotech (The Netherlands), secondary antibody, rabbit anti-rat IgG was obtained from Southern Biotech (Clinisciences; France).

Techniques: Injection, Isolation, Staining, Software

Journal: Circulation. Cardiovascular Imaging

Article Title: Spin Labeling-MRI Detects Increased Myocardial Blood Flow Post Endothelial Cell Transplantation in the Infarcted Heart

doi: 10.1161/CIRCIMAGING.111.966317

Figure Lengend Snippet: Slice-average and regional MBF at 1-day (inset of A) and 2-weeks post-MI (A). The MBF maps and corresponding LGE images were shown for a representative heart from EC (B–C, arrows pointing to the phantom) and Vehicle group (D–E). Immunostaining for vWF from an EC- (F) or Vehicle-treated heart (G). Double immunostaining of CD34 which reacts only to human tissue and vWF antibody which reacts to rat and human antigen revealed incorporation of ECs into capillaries in the infarcted (H) and border (I) region. Scale bars = 100 μm. MVD (= the number of capillaries per field of 1.1 mm2). *: statistically significant comparing the EC vs. Vehicle group (A, J).

Article Snippet: Sections were immunostained using the following antibodies: 1. rabbit polyclonal anti- von Willebrand Factor (vWF) antibody (Sigma, St. Louis, MO) and FITC-conjugated goat anti-rabbit secondary antibody, 2. mouse monoclonal anti-human CD34 antibody (Abcam, Cambridge, MA) and Cy3-conjugated goat anti-mouse secondary antibody.

Techniques: Immunostaining, Double Immunostaining

Journal: Molecular medicine reports

Article Title: Interleukin-17 potently increases non-small cell lung cancer growth.

doi: 10.3892/mmr.2015.4694

Figure Lengend Snippet: Figure 2. CD34 and CCR2 expression in F4/80-positive macrophages in implanted tumor masses analyzed by flow cytometry. At day 14, 16 and 18 after implantation, cells were obtained from the implanted tumor masses by dissection. (A) Cells were homogenized, stained with CD34 antibodies and examined by flow cytometry. (B) Quantification of results. A significantly increased proportion of CD34 in the IL-17 group compared with control. (C) Cells were stained with CCR2 antibodies and analyzed by flow cytometry. (D) CCR2-positive macrophages were increased in the IL-17 group compared with control. (*P<0.05, compared with control). IL, interleukin.

Article Snippet: Rat anti-mouse CD34 monoclonal antibody (mAb) (MEC 14.7, cat no. NB600-1071), phycoerythrin (PE)-conjugated rat antimouse F4/80 mAb (cat no. NBP2-22134), rat anti-mouse vascular endothelial growth factor (VEGF) mAb (RM0009-2G02, cat no. NB110-61025), rabbit anti-mouse matrix metalloproteinase (MMP)2 mAb (cat no. NB200-193), rabbit anti-mouse MMP9 mAb (cat no. NBP1-39597), rabbit anti-mouse tumor necrosis factor (TNF)-α mAb (cat no. NB100-57620) and mouse antimouse glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mAb (cat no. NB300-328H) were purchased from Novus Biologicals (Littleton, CO, USA).

Techniques: Expressing, Flow Cytometry, Dissection, Staining, Control